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KMID : 1094719970020020105
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 2 p.105 ~ p.108
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Isolation and characterization of soil strains producing glutaryl-7-aminocephalosporanic acid acylase
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Khang Yong-Ho
Yoo Byong-Hoon
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Abstract
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A search was undertaken to screen microorganisms that produce an enzyme capable of deacylating glutaryl-7-aminocephalosporanic acid to 7-aminocephalosporanic acid in soil samples. The screening was carried out by preparing enrichment cultures containing glutaryl-7ACA and cephalosporin C as selective carbon sources. A non-β-lactam model compound, glutaryl-p-nitroanilide, was synthesized as a substrate suitable for the rapid screening of microorganisms isolated from the enrichment cultures. Two isolates exhibiting acylase activity, designated BY7.4 and BY8.1, were identified as strains ofPseudomonas species.Pseudomonas BY8.1 showed higher acylase activity toward Gl-7ACA thanPseudomonas BY7.4. Environmental conditions for the optimal acylase activity ofPseudomonas BY8.1 were shown to be pH 9 and 30°C.
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KEYWORD
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screening, 7-ACA, glutaryl-7ACA, cephalosporin acylase, bioconversion
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